Rapid diagnosis of whooping cough using monoclonal antibody.

EVALUATION OF SERUM OPSONIC CAPACITY AGAINST BORDETELLA PERTUSSIS BY CHEMILUMINESCENCE

Serum opsonic capacity against B. pertussis was studied by using quantitative chemiluminescence (CL), a method known to have several advantages over conventional methods in evaluating opsonization and phagocytosis. Sera from unvaccinated infants was shown not to contain opsonins against B. pertussis and in unvaccinated infants suffering from whooping cough, no opsonins were detected. In ad...

Preparation and Evaluation of Stained Pertussis Antigen for Serodiagnosis of Whooping Cough

A Capture ELISA for the Diagnosis of Visceral Leishmaniasis Using a Monoclonal Antibody against a Leishmanial

capture ELISA system was developed for diagnosis of visceral leishmaniasis (VL) using a monoclonal antibody raised against an antigen previously detected in the urine of VL patients. Urine samples from confirmed VL cases from Yemen, Nepal, Spain, Sudan and Brazil were tested in the capture ELISA in comparison with urine samples from endemic and non-endemic areas along with urine samples from pa...

Development of monoclonal antibodies against axenic amastigotes of Leishmania infantum strain in Iran: Implication for diagnosis of Kala-azar

Objective(s): Leishmaniasis is endemic in 88 countries. Amastigote forms of Leishmania are experts at exploiting host cell processes to establish infection. Monoclonal antibodies are key reagents used in the diagnosis of infectious and non-infectious diseases. The aim of this study was to produce monoclonal antibodies against axenic amastigotes of the Leishmania infantum strain in Iran.Material...

Whooping cough in school age children with persistent cough: prospective cohort study in primary care.

OBJECTIVE To estimate the proportion of school age children with a persistent cough who have evidence of a recent Bordetella pertussis infection. DESIGN Prospective cohort study (October 2001 to March 2005). SETTING General practices in Oxfordshire, England. PARTICIPANTS 172 children aged 5-16 years who presented to their general practitioner with a cough lasting 14 days or more who conse...